Uma equipe de engenheiros da Johns Hopkins pode localizar cânceres agressivos usando luz laser e DNA dobrado
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Imagens Getty
Engenheiros da Johns Hopkins criaram uma nova ferramenta óptica que pode melhorar a imagem do câncer. A abordagem deles, chamada SPECTRA, usa pequenas nanosondas que acendem quando se ligam a células cancerígenas agressivas, ajudando os clínicos a distinguir entre cânceres localizados e aqueles que são metastáticos e têm o potencial de se espalhar por todo o corpo.
"Nossas descobertas mostram que o SPECTRA tem enorme potencial para detecção e geração de imagens de câncer", disse o líder da equipe Ishan Barman, professor de engenharia mecânica na Whiting School of Engineering. "Estamos dando aos clínicos uma ferramenta mais poderosa que pode encontrar células cancerígenas mais cedo e com mais precisão do que nunca."
A pesquisa da equipe aparece em Advanced Functional Materials .
Escalável e mais econômico do que os métodos atuais, o SPECTRA alavanca uma combinação inédita: espectroscopia Raman — que usa a dispersão de luz laser para fornecer informações detalhadas sobre vibrações moleculares — e origami de DNA, que envolve dobrar o DNA em formas específicas, como na arte japonesa de dobrar papel. A equipe usou o DNA dobrado como um arcabouço para criar nanopartículas plasmônicas precisamente organizadas, repórteres Raman (uma molécula que produz um sinal forte quando analisada usando espectroscopia Raman) e sequências de DNA direcionadas ao câncer. Essas nanosondas multifuncionais foram então testadas em células cancerígenas.
A equipe descobriu que, diferentemente de tomografias computadorizadas ou ressonâncias magnéticas que podem indicar a presença de um tumor, mas não as assinaturas moleculares específicas que podem alertar os médicos sobre metástases atuais ou iminentes, o SPECTRA se ligou de forma eficaz e consistente às células DU145 do câncer de próstata metastático e até mesmo as diferenciou das células não metastáticas.
Além disso, os pesquisadores selecionaram um repórter Raman — uma molécula de sinalização — que resulta em um sinal ativo e distinto em uma faixa que o destaca claramente contra o fundo do tecido normal, ajudando os médicos a localizar a doença com mais precisão.
"É um design inteligente que dá alto realce ao sinal Raman, e é uniforme", disse o membro da equipe Swati Tanwar, um bolsista de pós-doutorado em engenharia mecânica. "Ele pode distinguir células cancerígenas agressivas de não agressivas com base na intensidade do sinal. Em um tumor, se 10% das células são agressivas e 90% são não agressivas, as 10% acenderão e darão um sinal muito alto."
Tanwar disse que cada fita de DNA no andaime de origami tem uma sequência única e ocupa uma posição específica na nanoestrutura dobrada do origami. Esse arranjo meticuloso facilitou a criação da nanosonda multifuncional SPECTRA.
"A espectroscopia Raman é uma ferramenta de impressão digital molecular", disse o membro da equipe Lintong Wu, um estudante de doutorado em engenharia mecânica. "As moléculas podem parecer semelhantes à distância, mas usando a espectroscopia Raman elas mostram picos e sinais diferentes em todo o espectro."